Аннотация
В связи с тем, что в России скрининг рака шейки матки (РШМ) должен проводиться путём окрашивания мазков по Папаниколау целью данной работы было изучение вариантов протокола окраски по Папаниколау эксфолиативных вагинальных мазков путем обзора англоязычной и отечественной литературы. Метод регидратации высушенных на воздухе мазков с шейки матки с последующей фиксацией и окрашиванием может решить большинство проблем, связанных с влажной фиксацией биологического материала на предметном стекле, если окрашивание производят по Папаниколау. Существует несколько зарубежных исследований, посвященных регидратации мазков с шейки матки как альтернативный вариант влажной фиксации при нанесении мазков традиционным способом. В отечественной литературе работы по данной теме отсутствуют. Для достижения этой цели мы провели валидизацию методики в лаборатории с учетом материально-технического обеспечения и особенностей получения и доставки материала.
Регидратация с последующей фиксацией высушенных на воздухе мазков является простым, общедоступным, применимым и надежным методом фиксации, который сравним с традиционным методом влажной фиксации, используемым для мазков из шейки матки, и может применяться для рутинной оценки.
Annotation
Due to the fact that in Russia, screening for cervical cancer (CC) should be carried out by staining smears with Papanicolaou, the purpose of this work was to study options for the Papanicolaou staining protocol for exfoliative vaginal smears by reviewing English-language and domestic literature. The method of rehydrating air-dried cervical smears followed by fixation and staining can solve most of the problems associated with wet fixation of biological material on a glass slide if staining is performed using the Papanicolaou method. There are several foreign studies on the rehydration of cervical smears as an alternative to wet fixation when applying smears in the traditional way. There are no works on this topic in the domestic literature. To achieve this goal, we validated the method in the laboratory, taking into account logistics and the specifics of obtaining and delivering the material. Rehydration followed by fixation of air-dried smears is a simple, readily available, feasible and reliable fixation method that is comparable to the traditional wet fixation method used for cervical smears and can be used for routine evaluation.
Key words: cytological diagnostics; fixation of cytological samples; Papanicolaou staining.
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