Аннотация
Пробиотики представляют собой фармацевтические препараты, БАД или пищевые добавки с живыми бактериями, которые оказывают восстанавливающее влияние на здоровье человека, способствуют активации иммунной системы, улучшают течение аллергических состояний. Часто в качестве пробиотиков, используются бактерии Lactobacillus spp. Правильное употребление пробиотиков может быть эффективным при лечении дисбактериозов, диареи различного генеза, заболеваний полости рта, мочеполовой системы и т. п. Цель исследования: отбор аутоштаммов, которые могут быть использованы в качестве основы при создании пробиотических препаратов. Изучены 182 штамма Lactobacillus spp., из них отобраны девять: 9SM, Dec 1, Ku-f, 12 Pet-f, 14 Ul-d, 15 Sul-c, 10 POD, 7LV, Куз 2, которые идентифицированы до вида и внесены во Всероссийскую коллекцию промышленных микроорганизмов (БРЦ ВКПМ) (Москва). Все отобранные аутоштаммы лактобактерий обладают высоким уровнем перекрёстной адгезии, показали индекс адгезивности микроорганизмов (ИАМ)≥40,0 больше, чем у 50% пациентов. Лучшую адгезию показал аутоштамм 10 POD (ИАМ≥40,0 у 80% исследуемых пациентов). Все исследуемые штаммы Lactobacillus spp. устойчивы к ванкомицину, промежуточную устойчивость к антибиотику норфлоксацину имеют штаммы Ku-f, Куз 2, 15 Sul-c, устойчивыми оказались Dec 1 и 7LV. Исследуемые штаммы активно ингибируют рост E. coli и S. aureus, обладают высокой адгезионной способностью, имеют хорошую кислотообразующую и антагонистическую активности, что указывает на их высокую эффективность в качестве пробиотических бактерий. Показано, что рассматриваемые аутоштаммы Lactobacillus spp. могут быть определены как кандидаты для создания новых эффективных пробиотиков.
Annotation
Probiotics are pharmaceutical preparations, dietary supplements or nutritional supplements with live bacteria that have a restorative effect on human health, contribute to the activation of the immune system, and improve the course of allergic conditions. The bacteria most commonly used as probiotics are Lactobacillus spp. Proper use of probiotics can be effective in the treatment of dysbacteriosis, diarrhea of various origins, diseases of the oral cavity, genitourinary system, etc. The purpose of the study: the selection of autostrains that can be used as a basis for the creation of probiotic preparations. In our study, 182 strains of Lactobacillus spp. were studied, nine of them were selected: 9SM, Dec 1, 12 Pet-f, Ku-f, 14 Ul-d, 15 Sul-c, 10 POD, 7LV, Kuz 2, which were identified to species and included in the All-Russian Collection of Industrial Microorganisms (Moscow). All these autostrains of lactobacilli have a high level of cross-adhesion. They showed an index of microorganism adhesiveness (IAM)≥40.0 greater than in 50% of patients. The 10 POD autostrain showed the best adhesion (IAM≥40.0 in 80% of the studied patients). All studied strains of Lactobacillus spp. resistant to vancomycin, strains Ku-f, Kuz 2, 15 Sul-c have intermediate resistance to the antibiotic norfloxacin, and Dec 1 and 7LV turned out to be resistant. The studied strains actively inhibit the growth of E. coli and S. aureus, have a high adhesive ability, have good acid-forming and antagonistic activities, which indicates their high efficiency as probiotic bacteria. As a result of the research, it was shown that the examined autostrains of Lactobacillus spp. can be identified as candidates for the creation of new effective probiotics.
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