ОБЗОР НОВЫХ МЕТОДИЧЕСКИХ УКАЗАНИЙ ПО ЛАБОРАТОРНОЙ ДИАГНОСТИКЕ ДИФТЕРИЙНОЙ ИНФЕКЦИИ

Аннотация

Проанализирован обзор изменений, которые введены в новом нормативном методическом документе МУК 4.2.3852-23 «Лабораторная диагностика дифтерийной инфекции». Представлен новый алгоритм лабораторной диагностики дифтерийной
инфекции с учётом использования бактериологического метода, ПЦР-диагностики, серологических методов при обследовании с диагностической, профилактической целью и по эпидпоказаниям. Описан новый алгоритм бактериологического исследования с оценкой токсигенных свойств, постановкой тестов на цистиназную активность (проба Пизу), нитратредуктазную, уреазную, сахаролитическую активности с сывороточного агара. Проанализированы изменения по каждому разделу
нового методического документа.

Annotation

A review of the changes introduced in the new regulatory methodological document MUK 4.2.3852-23 “Laboratory Diagnosis of
Diphtheria Infection» was analyzed. A new algorithm for laboratory diagnostics of diphtheria infection was presented. New algorithm
for laboratory diagnostics of diphtheria infection includes use of bacteriological method, PCR diagnostics and serological methods for
examination with diagnostic, preventive purposes and epidemiological indications. A new algorithm of bacteriological examination
with assessment of toxigenic properties, setting tests for cystinase activity (Pizu probe), nitrate reductase, urease and sucrose activity
from serum agar is described. The changes for each section of the new methodological document were analyzed.
Key words: Corynebacterium diphtheriaе; bacteriological diagnostics; PCR diagnostics; diphtheria infection; algorithm

Об авторах

Список литературы

1. Instruction on bacteriological research for diphtheria from 05.03.1951,
USSR Ministry of Health; 1951. (in Russian)
2. Instruction for Bacteriological Diphtheria № 303-59 dated 19.09.1959.
USSR Ministry of Health; 1959. (in Russian)
3. Instruction for Bacteriological Diphtheria № 690-67 dated 02.09.1967.
USSR Ministry of Health; 1967. (in Russian)
4. Instruction on bacteriological testing for diphtheria (Appendix 4 to
Order No. 580 of the Ministry of Health of the USSR of 26.06.1974).
USSR Ministry of Health; 1974. (in Russian)
5. Bacteriological studies in case of diphtheria infection (methodological recommendations) from 10.04.1980. Ministry of Health of the RSFSR; 1980. (in Russian)
6. Instruction on Bacteriological and Serological Indication of the Agent of
Diphtheria and its Toxin (Appendix 5 to Order № 450 of the Ministry of
Health of the USSR of 02.04.1986). Minzdrav USSR; 1986. (in Russian)
7. Methodological Guidelines «Laboratory Diagnostics of Diphtheria
Infection» dated 09.01.1998, MUK 4.2.698-98. Ministry of Health of
Russia. Moscow: «Intersen»; 1998. ISBN 5-89834-02-3. (in Russian)
8. Methodological Guidelines «Laboratory Diagnostics of Diphtheria
Infection» dated 14.07.2013, MUK 4.2.3065-13. Rospotrebnadzor;
2013. ISBN 978-5-7508-1195-3. (in Russian)
9. Methodological Recommendations for the Use of Modified Pizu Medium and Indicator Paper Discs for Identification, Biochemical Typing and Determination of Toxicity of Diphtheria Microbes № 28-6/31.
USSR Ministry of Health; 1989. (in Russian)

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